Effect of chromophore encapsulation on linear and nonlinear optical properties: the case of "miniSOG", a protein-encased flavin.

摘要: Linear and nonlinear spectroscopic parameters of flavin mononucleotide, FMN, have been examined both experimentally computationally under conditions in which FMN is (1) solvated a buffered aqueous solution, (2) encased protein that likewise solution. The latter was achieved using “miniSOG” an FMN-containing engineered from Arabidopsis thaliana phototropin 2. Although it reasonable to expect the encasing could appreciable effect, certainly on two-photon absorption cross section, we find replacing dynamic environment with more static does little influence properties FMN. experimental computational studies are consistent this regard, agreement indicates comparatively high-level methods can indeed be used success large chromophores complicated local environment. results present study facilitate much-needed development well-characterized readily-controlled suitable for use as intracellular sensitizers fluorophores.