The use of miniSOG in the localization of mitochondrial proteins.
作者： Guy A. Perkins
DOI: 10.1016/B978-0-12-801415-8.00010-2
关键词:
摘要: In this chapter, we provide details along with considerations and future directions for the use of miniSOG (for mini Singlet Oxygen Generator), a versatile label correlated light electron microscopy genetically tagged proteins in cells, tissues, organisms. This new visualizable genetic tag improves ability biologists to locate specific at nanoscale resolution see these environment structural landmarks that are used navigating by, such as mitochondrial membranes compartments. provides high-quality ultrastructural preservation permits three-dimensional protein localization via tomography or serial section block-face scanning microscopy. is now doing what family green fluorescent did fluorescence
elsevier.com
sci-hub.se 参考文章(13)
Mark H. Ellisman, Thomas J. Deerinck, Xiaokun Shu, Gina E. Sosinsky, Picking Faces out of a Crowd Methods in Cell Biology. ,vol. 111, pp. 139- 155 ,(2012) , 10.1016/B978-0-12-416026-2.00008-X
Nanna H. List, Frederico M. Pimenta, Lotte Holmegaard, Rasmus L. Jensen, Michael Etzerodt, Tobias Schwabe, Jacob Kongsted, Peter R. Ogilby, Ove Christiansen, Effect of chromophore encapsulation on linear and nonlinear optical properties: the case of "miniSOG", a protein-encased flavin. Physical Chemistry Chemical Physics. ,vol. 16, pp. 9950- 9959 ,(2014) , 10.1039/C3CP54470B
Joshua M. Baughman, Fabiana Perocchi, Hany S. Girgis, Molly Plovanich, Casey A. Belcher-Timme, Yasemin Sancak, X. Robert Bao, Laura Strittmatter, Olga Goldberger, Roman L. Bogorad, Victor Koteliansky, Vamsi K. Mootha, Integrative genomics identifies MCU as an essential component of the mitochondrial calcium uniporter Nature. ,vol. 476, pp. 341- 345 ,(2011) , 10.1038/NATURE10234
John Y. Lin, Sharon B. Sann, Keming Zhou, Sadegh Nabavi, Christophe D. Proulx, Roberto Malinow, Yishi Jin, Roger Y. Tsien, Optogenetic inhibition of synaptic release with chromophore-assisted light inactivation (CALI). Neuron. ,vol. 79, pp. 241- 253 ,(2013) , 10.1016/J.NEURON.2013.05.022
Alexander Ludwig, Gillian Howard, Carolina Mendoza-Topaz, Thomas Deerinck, Mason Mackey, Sara Sandin, Mark H. Ellisman, Benjamin J. Nichols, Molecular Composition and Ultrastructure of the Caveolar Coat Complex PLoS Biology. ,vol. 11, pp. e1001640- ,(2013) , 10.1371/JOURNAL.PBIO.1001640
Andrew P. Wojtovich, Thomas H. Foster, Optogenetic control of ROS production. Redox biology. ,vol. 2, pp. 368- 376 ,(2014) , 10.1016/J.REDOX.2014.01.019
Xiaokun Shu, Varda Lev-Ram, Thomas J. Deerinck, Yingchuan Qi, Ericka B. Ramko, Michael W. Davidson, Yishi Jin, Mark H. Ellisman, Roger Y. Tsien, A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms. PLOS Biology. ,vol. 9, ,(2011) , 10.1371/JOURNAL.PBIO.1001041
Marcus Wingen, Janko Potzkei, Stephan Endres, Giorgia Casini, Christian Rupprecht, Christoph Fahlke, Ulrich Krauss, Karl-Erich Jaeger, Thomas Drepper, Thomas Gensch, The photophysics of LOV-based fluorescent proteins – new tools for cell biology Photochemical and Photobiological Sciences. ,vol. 13, pp. 875- 883 ,(2014) , 10.1039/C3PP50414J
Margaret T Butko, Jin Yang, Yang Geng, Hyung Joon Kim, Noo Li Jeon, Xiaokun Shu, Mason R Mackey, Mark H Ellisman, Roger Y Tsien, Michael Z Lin, Fluorescent and photo-oxidizing TimeSTAMP tags track protein fates in light and electron microscopy Nature Neuroscience. ,vol. 15, pp. 1742- 1751 ,(2012) , 10.1038/NN.3246
Diego De Stefani, Anna Raffaello, Enrico Teardo, Ildikò Szabò, Rosario Rizzuto, A forty-kilodalton protein of the inner membrane is the mitochondrial calcium uniporter Nature. ,vol. 476, pp. 336- 340 ,(2011) , 10.1038/NATURE10230