Interactions of monocytic cells with human endothelial cells stimulate monocytic metalloproteinase production.

摘要: Monocyte-endothelial cell interactions play an important role in the early stages of atherosclerosis, and it is hypothesized that regulation metalloproteinase production by these contributes to this pathological process. The effects monocytic cell-endothelial on were investigated using vitro system, focusing endothelial secretions physical contact as effectors expression. Human umbilical vein cells (HUVECs) human line THP-1 used, changes levels secretion mRNA measured. When incubated for 18 hours with HUVEC conditioned medium (CM), a four- eightfold induction MMP-9 was observed at both protein levels; however, another metalloproteinase, MMP-2, unaffected. CM confirmed freshly isolated monocytes. A sevenfold increase apically collected but not basally CM. paraformaldehyde-fixed HUVECs plasma membranes showed levels, measurements activity found due either or threefold increase. molecular weight secreted effector molecule(s) determined be 30 +/- 6 kd. data show through release soluble factors direct regulate production, which has implications atherogenic