Suppression of Wnt signaling by the green tea compound (-)-epigallocatechin 3-gallate (EGCG) in invasive breast cancer cells. Requirement of the transcriptional repressor HBP1.

摘要: Genetic and biochemical de-regulation of Wnt signaling is correlated with breast other cancers. Our goal was to identify compounds that block as a first step toward investigating new strategies for suppression invasive In limited phytonutrient screen, EGCG ((–)-epigallocatechin 3-gallate), the major phytochemical in green tea, emerged an intriguing candidate. Epidemiological studies have associated tea consumption reduced recurrence inhibited by dose-dependent manner cancer cells. The apparent mechanism targeted HBP1 transcriptional repressor, which we had previously characterized suppressor signaling. treatment induced repressor levels through increase mRNA stability, but not initiation. To test functionality, DNA-based short hairpin RNA (shRNA) used knockdown endogenous gene. Consistently, lines sensitivity target gene (c-MYC). Because our ongoing clinically link abrogation cancer, tested if also regulated biological functions de-regulated cancer. both cell tumorigenic proliferation invasiveness HBP1-dependent manner. Together, emerging blocks inducing inhibits aspects These provide framework considering future prevention.