Transglycosylation of a β-galactosyl radical, in the course of enzymic hydrolysis of lactose, in the presence of selected polyhydroxyalcohols.

作者: Robert Klewicki

DOI: 10.1023/A:1005698013715

关键词:

摘要: Sorbitol, xylitol, erythritol and lactitol were used as the acceptors of galactosyl radicals, in process transgalactosylation accompanying hydrolysis lactose, conducted with β-galactosidase (4.0 ml Lactozym 3000 was added to 430 1.45 M lactose 0.95 polyhydroxyalcohols). The following concentrations derivatives polyols obtained after for 4 h at 40 °C: 0.31 Gal-erythritol, 0.22 Gal-xylitol, 0.18 Gal-sorbitol 0.14 Gal-lactitol. A quadruple increase xylitol content dry matter a solution (from 11.5% (w/w) 44.5% brought about 2.3-fold product (15.2% matter).

参考文章(9)
Ken-ichi Iwasaki, Mitsutoshi Nakajima, Shin-ichi Nakao, Galacto-oligosaccharide production from lactose by an enzymic batch reaction using β-galactosidase Process Biochemistry. ,vol. 31, pp. 69- 76 ,(1996) , 10.1016/0032-9592(94)00067-0
J. L. Berger, B. H. Lee, C. Lacroix, Oligosaccharides synthesis by free and immobilized β-galactosidases from Thermus aquaticus YT-1 Biotechnology Letters. ,vol. 17, pp. 1077- 1080 ,(1995) , 10.1007/BF00143104
J. E. Prenosil, E. Stuker, J. R. Bourne, Formation of oligosaccharides during enzymatic lactose hydrolysis and their importance in a whey hydrolysis process: Part II: Experimental. Biotechnology and Bioengineering. ,vol. 30, pp. 1026- 1031 ,(1987) , 10.1002/BIT.260300905
Bernd Sauerbrei, Joachim Thiem, Galactosylation and glucosylation by use of β-galactosidase Tetrahedron Letters. ,vol. 33, pp. 201- 204 ,(1992) , 10.1016/0040-4039(92)88050-F
J. E. Prenosil, E. Stuker, J. R. Bourne, Formation of oligosaccharides during enzymatic lactose: Part I: State of art. Biotechnology and Bioengineering. ,vol. 30, pp. 1019- 1025 ,(1987) , 10.1002/BIT.260300904
David E. Stevenson, Roger A. Stanley, Richard H. Furneaux, Optimization of alkyl β‐D‐galactopyranoside synthesis from lactose using commercially available β‐galactosidases Biotechnology and Bioengineering. ,vol. 42, pp. 657- 666 ,(1993) , 10.1002/BIT.260420514
O K.K. Yakult Honsha Matsumoto, Tatsuhiko C, O K.K. Yakult Honsha Kan, Yoichi C, O K.K. Yakult Honsha Kobayashi, Keisuke C, Method of preparing galactooligosaccharides ,(1989)
Horikoshi Koki, Kubota Shinobu, Nakamura Nobuyuki, Ogawa Koichi, PRODUCTION OF GALACTOSYL-MALTOOLIGOSACCHARIDE DERIVATIVE ,(1994)