Nonenzymatic glycosylation of peripheral nerve protein in diabetes mellitus

摘要: A new affinity chromatography system that selectively retains glycosylated amino acids has been utilized to determine the amount of nonenzymatic glycosylation present in peripheral nerve from diabetic and control rats dogs. The mean value for was 2.8 times greater than normal (P less 0.001). In dogs, values were 2.15 0.05). Amino acid analysis reduced, previously isolated by showed lysine its hydrolysis rearrangement products major borohydride-reducible adduct present. addition, another product noted be proportions. This radioactive did not chromatograph with any available standards. finding diabetes results a nearly 3-fold increase is consistent number previous investigations which measured hemoglobin, serum albumin, urinary peptides diabetics normals. reported here provide evidence increased occurring tissue where physiological, morphological, clinical degeneration characteristically develop as result mellitus.