GLUT1 glucose transporter expression in the diabetic and nondiabetic human eye

摘要: Purpose. The GLUT1 glucose transporter is expressed in endothelial and epithelial barriers, including the retinal capillary endothelium pigment epithelium (RPE) of eye. present studies were undertaken to determine whether additional cell types within human eye aberrantly diabetic proliferative retinopathy humans. Methods. Immunohisiochemical staining sections eyes obtained at surgery or autopsy from patients with without diabetes was performed polyclonal antisera directed against transporter. Results. In course this study, an unexpected multicellular localization different cellular barriers observed. nondiabetic eye, specific for seen nerve fiber layer, ganglion photoreceptor bodies, capillaries RPE retina, basal infoldings pigmented nonpigmented layers ciliary body, cndoihelium posterior iris, corneal endothelium, lining canal Schlemm. Miiller cells, a type glial identified by morphology parallel fibrillary acidic protein, also stained intensely positive GLUT1. pattern immunoreactivity virtually identical that specimens, notable exception neovascular did not stain Conclusions. These describe heretofore unrecognized expression immunoreactive layer Schlemm, cells study provides evidence central nervous system. Because characteristic tissues possess barrier function, absence GLUT! tissue suggests loss selective permeability associated facilitated transport disorder. Invest Ophthalmol Vis Sci. J994;35:2887-2894.