D-Pinitol treatment induced the apoptosis in human leukemia MOLT-4 cells by improved apoptotic signaling pathway.
作者： Xiangmei Yao , Keqian Shi , Yanmei Yang , Xuezhong Gu , Weiwei Tan
DOI: 10.1016/J.SJBS.2020.06.034
关键词:
摘要: Abstract Cancer is still remain as a global burden with the 18.1 million and 9.6 new cases mortlities, respectively estimated globally. Leukemia may arise at all ages varied from infants to elders. In this exploration, we planned evaluate antiproliferative effect of D-pinitol on human leukemia MOLT-4 cells. Anticancer potential was examined using MTT assay. Reactive oxygen species (ROS) generation studied by fluorescence microscopic method DCFH-DA staining. Apoptotic morphological alterations were determined dual staining (acridine orange ethidium bromide). Western blot ELISA methods employed study apoptotic protein expression. treatment significantly induced cytotoxicity in We observed that induces ROS Further, noticed apoptosis dosage dependent manner. Moreover, western based analysis revealed elevated Bax, Caspase-3, Caspase-9 attenuated Bcl-2 expression leukemic cancer cell. Our findings suggest cells generating intracellular modulating
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