Smad2/3-Regulated Expression of DLX2 Is Associated with Radiation-Induced Epithelial-Mesenchymal Transition and Radioresistance of A549 and MDA-MB-231 Human Cancer Cell Lines

摘要: The control of radioresistance and metastatic potential surviving cancer cells is important for improving eradication by radiotheraphy. distal-less homeobox2 (DLX2) gene encodes a homeobox transcription factor involved in morphogenesis its deregulation was found human solid tumors hematologic malignancies. Here we investigated the role DLX2 association with radiation-induced epithelial to mesenchymal transition (EMT) stem cell-like properties regulation Smad2/3 signaling irradiated A549 MDA-MB-231 cell lines. In cells, EMT induced as demonstrated marker expression, phosphorylation Smad2/3, migratory invasive ability. Also, showed increased (CSCs) marker. Interestingly, overexpressed upon irradiation. Therefore, examined radioresistance. overexpression alone EMT, migration invasion, CSC expression. reduced colony-forming ability partially restored overexpression. On other hand, depletion using si-RNA abolished cells. radiation sensitivity both Moreover, knockdown key activator TGF-β1 pathway, abrogated indicating that expression dependent on signaling. These results plays crucial radioresistance, regulated