The canine oocyte penetration assay; its use as an indicator of dog spermatozoal performance in vitro

摘要: Abstract In vitro maturation and fertilisation has yet to be thoroughly investigated in the dog is work that required before gamete salvage programmes can established endangered canine species. Due differences which exist between reproductive function of Canidae other domestic species, requirements both oocyte spermatozoa may also differ, these remain established. The objective this study was investigate ability capacitated penetrate zona pellucida matured oocytes. Two methods, one utilised fluorescent nuclear label Hoechst 33258 combination with an aceto–orcein stain light microscopy, another using fluorescence microscopy method alone were effective for staining material penetrated spermatozoal heads. These techniques only rarely allowed identification same oocyte. Using assay, two Tyrodes media compared their allow capacitate acrosome react as measured by chlortetracycline dual method. No correlation however found acrosomal status penetration homologous oocytes, although some relationship observed. addition, it effect stage had no upon immature oocytes could