Effects of meiosis-inhibiting agents and equine chorionic gonadotropin on nuclear maturation of canine oocytes

摘要: Experiments were conducted to determine the effects of meiosis-inhibiting-agents and gonadotropins on nuclear maturation canine oocytes. The culture medium was TCM199 + 10 ng/ml epidermal growth factor supplemented with 25 μM β-mercaptoethanol, 0.25 mM pyruvate, 1.0 L-glutamine (Basal TCM). Initially, oocytes cultured in Basal TCM alone or TCM + dibutylryl cyclic adenosine monophosphate (0.5, 1, 5, 10 dbcAMP) for 24 hr. Dibutylryl cAMP inhibited resumption meiosis a dose-dependent manner; 60% remained at germinal vesicle (GV) stage after being hr 5 dbcAMP. meiosis-inhibitory effect dbcAMP appeared be reversible, as resumed completed an additional 48 its absence. Oocytes then TCM + roscovitine (12.5, 25, 50 μM) Although ∼60% roscovitine GV stage, this percentage not significantly different from 48% that also when TCM + 17 hr, exposed briefly equine chorionic gonadotropin (eCG), Short exposure eCG beneficial, it increased proportion developing beyond breakdown (P < 0.05) ∼20–30% these metaphase I (MI) Study kinetics demonstrated large numbers MI even 52 following brief eCG. This study showed vitro can somewhat improved by short However, further studies are still required derive effective methods mature vitro. Mol. Reprod. Dev. 65: 435–445, 2003. © 2003 Wiley-Liss, Inc.