HIF-1 alpha inhibition ameliorates neonatal brain damage after hypoxic-ischemic injury.
作者： Wanqiu Chen , Vikram Jadhav , Jiping Tang , John H. Zhang
DOI: 10.1007/978-3-211-85578-2_77
关键词:
摘要: BACKGROUND Hypoxia-inducible-factor-1alpha (HIF-1alpha) has been considered as a regulator of both prosurvival and prodeath pathways in the nervous system. This study was designed to elucidate role HIF-1alpha neonatal hypoxia-ischemia (HI) brain injury. METHODS 2-methoxyestradiol (2ME2), inhibitor, tested at different dosages (1.5, 15 150 mg/kg) therapeutic window by administrating (15 immediately or 3 hours after induction hypoxic ischemic Infarct size using TTC staining edema were measured 48 post hypoxia-ischemia. Blood-brain barrier (BBB) permeability examined IgG staining. Vascular endothelial growth factor (VEGF) expression distribution studied immunohistochemistry western blotting analysis. FINDINGS 2ME2 exhibited dose-dependent neuroprotection decreasing infarct volume attenuating edema. also attenuated BBB disruption, decreased vascular expression. The neuroprotection, however, lost when administered HI. CONCLUSION shows that acute inhibition is neuroprotective hypoxic-ischemic injury preserving integrity reducing
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sci-hub.st 参考文章(15)
Peter Carmeliet, Yuval Dor, Jean-Marc Herbert, Dai Fukumura, Koen Brusselmans, Mieke Dewerchin, Michal Neeman, Françoise Bono, Rinat Abramovitch, Patrick Maxwell, Cameron J. Koch, Peter Ratcliffe, Lieve Moons, Rakesh K. Jain, Désiré Collen, Eli Keshet, Role of HIF-1alpha in hypoxia-mediated apoptosis, cell proliferation and tumour angiogenesis. Nature. ,vol. 394, pp. 485- 490 ,(1998) , 10.1038/28867
Marc W. Halterman, Craig C. Miller, Howard J. Federoff, Hypoxia-Inducible Factor-1α Mediates Hypoxia-Induced Delayed Neuronal Death That Involves p53 The Journal of Neuroscience. ,vol. 19, pp. 6818- 6824 ,(1999) , 10.1523/JNEUROSCI.19-16-06818.1999
Robert C Vannucci, Experimental Biology of Cerebral Hypoxia-Ischemia: Relation to Perinatal Brain Damage Pediatric Research. ,vol. 27, pp. 317- 326 ,(1990) , 10.1203/00006450-199004000-00001
J YAN, C CHEN, J LEI, L YANG, K WANG, J LIU, C ZHOU, 2-methoxyestradiol reduces cerebral vasospasm after 48 hours of experimental subarachnoid hemorrhage in rats. Experimental Neurology. ,vol. 202, pp. 348- 356 ,(2006) , 10.1016/J.EXPNEUROL.2006.06.009
Tamiji Tsubokawa, Mitsuo Yamaguchi-Okada, John W. Calvert, Ihsan Solaroglu, Norihito Shimamura, Kenichiro Yata, John H. Zhang, Neurovascular and neuronal protection by E64d after focal cerebral ischemia in rats. Journal of Neuroscience Research. ,vol. 84, pp. 832- 840 ,(2006) , 10.1002/JNR.20977
Zheng Gang Zhang, Li Zhang, Quan Jiang, Ruilan Zhang, Kenneth Davies, Cecylia Powers, Nicholas van Bruggen, Michael Chopp, VEGF enhances angiogenesis and promotes blood-brain barrier leakage in the ischemic brain Journal of Clinical Investigation. ,vol. 106, pp. 829- 838 ,(2000) , 10.1172/JCI9369
Dali Yin, Changman Zhou, Ikuyo Kusaka, John W. Calvert, Andrew D. Parent, Anil Nanda, John H. Zhang, Inhibition of Apoptosis by Hyperbaric Oxygen in a Rat Focal Cerebral Ischemic Model Journal of Cerebral Blood Flow and Metabolism. ,vol. 23, pp. 855- 864 ,(2003) , 10.1097/01.WCB.0000073946.29308.55
J. Althaus, M. Bernaudin, E. Petit, J. Toutain, O. Touzani, A. Rami, Expression of the gene encoding the pro-apoptotic BNIP3 protein and stimulation of hypoxia-inducible factor-1α (HIF-1α) protein following focal cerebral ischemia in rats Neurochemistry International. ,vol. 48, pp. 687- 695 ,(2006) , 10.1016/J.NEUINT.2005.12.008
Rob Helton, Jiankun Cui, John R Scheel, Julie A Ellison, Chris Ames, Claire Gibson, Barbara Blouw, Ling Ouyang, Ioannis Dragatsis, Scott Zeitlin, Randall S Johnson, Stuart A Lipton, Carrolee Barlow, Brain-Specific Knock-Out of Hypoxia-Inducible Factor-1α Reduces Rather Than Increases Hypoxic-Ischemic Damage The Journal of Neuroscience. ,vol. 25, pp. 4099- 4107 ,(2005) , 10.1523/JNEUROSCI.4555-04.2005
John E. Rice, Robert C. Vannucci, James B. Brierley, The influence of immaturity on hypoxic-ischemic brain damage in the rat Annals of Neurology. ,vol. 9, pp. 131- 141 ,(1981) , 10.1002/ANA.410090206