Inhibition of Apoptosis by Hyperbaric Oxygen in a Rat Focal Cerebral Ischemic Model

摘要: The hypothesis was tested that hyperbaric oxygen therapy (HBO) reduced brain infarction by preventing apoptotic death in ischemic cortex a rat model of focal cerebral ischemia. Male Sprague-Dawley rats were subjected to middle artery occlusion/reperfusion (MCAO/R) and subsequently exposed HBO (2.5 atmospheres absolute) for 2 h, at 6 h after reperfusion. Rats killed samples collected 24, 48, 72 7 days Neurologic deficits, area, changes evaluated clinical scores, 2,3,7-triphenyltetrazolium chloride staining, caspase-3 expression, DNA fragmentation assay, terminal deoxynucleotidyl transferase-mediated 2'-deoxyuridine 5'-triphosphate-biotin nick end labeling (TUNEL)-hematoxylin eosin (H&E) costaining. In MCAO/R without treatment animals, observed injured but not Double slides with NeuN demonstrated neurons the labeled caspase-3. TUNEL+H&E costaining revealed morphologic Hyperbaric abolished number TUNEL-positive cells. infarct area improved neurologic scores One molecular mechanisms HBO-induced protection is prevent apoptosis, this effect might preserve more tissues promote functional recovery.