Targeted toxicants to dopaminergic neuronal cell death.

摘要: Parkinson's disease (PD ) is mainly characterized by a progressive degeneration of dopaminergic neurons in the substantia nigra resulting chronic deficits motor functions. Administration neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP produces PD symptoms and recapitulates main features human animal models. MPTP converted to 1-methyl-4-phenylpyridine (MPP+ ), which active toxic compound that selectively destroys neurons. Here, we describe methods protocols evaluate MPTP/MPP+-induced neurodegeneration both murine primary mesencephalic cultures The ability MPTP/MPP+ cause neuronal cell death assessed immunostaining tyrosine hydroxylase (TH).