Cystine uptake and glutathione level in fetal brain cells in primary culture and in suspension.

摘要: — The glutathione level and the factors affecting this were investigated in fetal rat brain cells a primary culture. Early culture, of decreased, but after 5 h it began to increase. This increase was not observed cystine-free medium prevented by excess glutamate. Cystine taken up freshly isolated cell suspensions, its rate increased during cystine uptake mediated Na+-independent, glutamate-sensitive route previously found various types designated as system x−c. is crucial factor maintaining under because provides cysteine for synthesis. Cysteine undetectable before appeared, though at very low level, when cultured there. source medium. Presumably decrease early stage culture resulted from fact that did contain cysteine. enhancement may constitute protective mechanism against oxidative stress which are exposed. Regulation vivo transport discussed.