Purification of mitochondrial RNA polymerase from Saccharomyces cerevisiae.
作者： D. Levens , A. Lustig , M. Rabinowitz
DOI: 10.1016/S0021-9258(19)69987-4
关键词:
摘要: The RNA polymerase from the mitochondria of Saccharomyces cerevisiae has been extensively purified by Sepharose 4B, heparin 4B phosphocellulose, and DEAE-Sephadex A-50 chromatography. activity co-sediments with a 45,000-dalton polypeptide at 6.3 S in glycerol gradients. is inhibited antibodies to polypeptide. not rifampicin or alpha-amanitin. It requires Mg2+ elevated ionic strength Mn2+. most efficient template for poly[d(AT)], mtDNA being preferred natural template. transcribes petite strain F11 nonrandom manner.
sci-hub.st 参考文章(50)
R. Morimoto, J. Locker, R.M. Synenki, M. Rabinowitz, Transcription, processing, and mapping of mitochondrial RNA from grande and petite yeast. Journal of Biological Chemistry. ,vol. 254, pp. 12461- 12470 ,(1979) , 10.1016/S0021-9258(19)86337-8
Gianni Piperno, Godeleine Fonty, Giorgio Bernardi, The mitochondrial genome of wild-type yeast cells: II. Investigations on the compositional heterogeneity of mitochondrial DNA Journal of Molecular Biology. ,vol. 65, pp. 191- 205 ,(1972) , 10.1016/0022-2836(72)90276-8
Joseph Locker, Murray Rabinowitz, [1] An overview of mitochondrial nucleic acids and biogenesis Methods in Enzymology. ,vol. 56, pp. 3- 16 ,(1979) , 10.1016/0076-6879(79)56004-2
Pierre Chambon, 9. Eucaryotic RNA Polymerases The Enzymes. ,vol. 10, pp. 261- 331 ,(1974) , 10.1016/S1874-6047(08)60141-9
D. Levens, R. Morimoto, M. Rabinowitz, Mitochondrial transcription complex from Saccharomyces cerevisiae Journal of Biological Chemistry. ,vol. 256, pp. 1466- 1473 ,(1981) , 10.1016/S0021-9258(19)69986-2
Gary R. Craven, Edward Steers, Christian B. Anfinsen, Purification, Composition, and Molecular Weight of the β-Galactosidase of Escherichia coli K12 Journal of Biological Chemistry. ,vol. 240, pp. 2468- 2477 ,(1965) , 10.1016/S0021-9258(18)97348-5
J M Buhler, F Iborra, A Sentenac, P Fromageot, Structural studies on yeast RNA polymerases. Existence of common subunits in RNA polymerases A(I) and B(II). Journal of Biological Chemistry. ,vol. 251, pp. 1712- 1717 ,(1976) , 10.1016/S0021-9258(17)33707-9
David J. Shapiro, John M. Taylor, G. Stanley McKnight, Rafael Palacios, Carmen Gonzalez, Mary L. Kiely, Robert T. Schimke, Isolation of Hen Oviduct Ovalbumin and Rat Liver Albumin Polysomes by Indirect Immunoprecipitation Journal of Biological Chemistry. ,vol. 249, pp. 3665- 3671 ,(1974) , 10.1016/S0021-9258(19)42526-X
P.A. Tekamp, P. Valenzuela, T. Maynard, G.I. Bell, W.J. Rutter, Specific gene transcription in yeast nuclei and chromatin by added homologous RNA polymerases I and II. Journal of Biological Chemistry. ,vol. 254, pp. 955- 963 ,(1979) , 10.1016/S0021-9258(17)37897-3
Giorgio Bernardi, Gianni Piperno, Godeleine Fonty, The mitochondrial genome of wild-type yeast cells. I. Preparation and heterogeneity of mitochondrial DNA. Journal of Molecular Biology. ,vol. 65, pp. 173- 189 ,(1972) , 10.1016/0022-2836(72)90275-6