Structural studies on yeast RNA polymerases. Existence of common subunits in RNA polymerases A(I) and B(II).

摘要: The subunits of yeast RNA polymerases A(I) and B(II) were characterized using several techniques. present studies demonstrate that the A B enzymes possess three subunits, which are indistinguishable on basis molecular weight, isoelectric point, fingerprint pattern. common belong to small weight components enzymes. By polyacrylamide gel electrophoresis with sodium dodecyl sulfate they migrate apparent weights 27,000, 23,000, 14,500, respectively. two-dimensional subunit mapping technique was used separate according point weight. polypeptides co-migrated spots corresponding points 9.2 (27,000), 4.5 (23,000), 4.6 (14,500). fingerprints 35S-labeled tryptic peptides presumptive found be essentially identical. Finally, presence supported by fact antibodies against pure polymerase cross-react inhibit B. Except for it is likely primarily made distinct gene products following reasons. total 13 polypeptide chains in enzyme A, whereas 10 sulfur content majority these different two No similarity 35S-peptide from a number slightly largest do not or data discussed terms structural organization eukaryotic polymerases.