Oxidized ApoC1 on MALDI-TOF and glycated-ApoA1 band on gradient gel as potential diagnostic tools for atherosclerotic vascular disease.

摘要: Abstract Background Atherosclerotic vascular disease (ASVD), including coronary artery disease, ischemic stroke, and peripheral is the most common cause of death both in general population high-risk patients; patients with diabetes mellitus (DM), uremia (UM), primary hyperlipidemia (HP) have increased risks for developing ASVD. Methods To identify new biomarkers early prediction ASVD, HDL samples from (ASVD) but no documented ASVD (non-ASVD) were collected Bis-Tris gradient gel MALDI-TOF (matrix-assisted laser desorption ionization-time flight) analyses. Results Oxidation ApoC1 was detected specifically by MALDI-TOF. On gel, non-ASVD ApoA1 displayed into 2 distinct bands A B. An additional C band appeared exclusively patients. The extraordinary characterized high levels glycation oxidation. analyses peptides after trypsin digestion confirmed higher oxidation than samples. Conclusions Gel identification highly-glycated oxidized detection may provide a approach diagnosis.