Structure of the A Site of Escherichia coli 16S Ribosomal RNA Complexed with an Aminoglycoside Antibiotic

摘要: Aminoglycoside antibiotics that bind to 30S ribosomal A-site RNA cause misreading of the genetic code and inhibit translocation. The aminoglycoside antibiotic paromomycin binds specifically an oligonucleotide contains subunit A site, solution structure RNA-paromomycin complex was determined by nuclear magnetic resonance spectroscopy. in major groove model within a pocket created A-A base pair single bulged adenine. Specific interactions occur between chemical groups important for activity conserved nucleotides RNA. explains binding diverse aminoglycosides ribosome, their specific against prokaryotic organisms, various resistance mechanisms, provides insight into ribosome function.