Lactobacillus plantarum KFY02 enhances the relieving effect of gardenoside on montmorillonite induced constipation in mice

摘要: Lactobacillus plantarum KFY02 (KFY02), isolated from naturally fermented milk yoghurt in Korla, Xinjiang, Northwest of China, showed gardenoside action for the intestinal regulation constipated mice. Comparatively, effects (0.5 × 108 CFU kg−1, by body weight (BW)), (50 mg BW), and BW) + on mice with montmorillonite-induced constipation were also studied. Enzyme linked immunoassay, hemotoxylin eosin (H&E) staining, quantitative polymerase chain reaction (qPCR) assay high performance liquid chromatography (HPLC) analysis used study. Compared model group, genipin (combined group) increased propelling rate activated carbon small intestines accelerated discharge first black stool At same time, combination group reduced levels motilin (MTL), substance P (SP) endothelin-1 (ET-1) serum, somatostatin (SS), vasoactive peptide (VIP), acetylcholinesterase (AchE) gastrin (Gastrin) which made these parameters close to those normal group. Using qPCR analysis, it was observed that combined up-regulated mRNA expression endothelial nitric oxide synthase (eNOS), stem cell factor (SCF), receptor (c-Kit), glutathione (GSH), catalase manganese-superoxide dismutase (Mn-SOD) down-regulated inducible (iNOS) transient potential vanilloid 1 (TRPV1). The Bacteroides Akkermansia abundances decreased Firmicutes abundance feces Firmicutes/Bacteroides ratio. above genes similar results indicate produced β-glucosidase hydrolyze glycosidic bond produce can effectively promote gastrointestinal hormones peristalsis reduce oxidative tissue damage This study confirmed has relieving