Simultaneous analysis of host and pathogen interactions during an in vivo infection reveals local induction of host acute phase response proteins, a novel bacterial stress response, and evidence of a host-imposed metal ion limited environment.

摘要: Summary A fundamental goal in the study of infections is to understand dynamic interplay between host and pathogen; however, direct vivo interrogation this disease process via transcriptional profiling has been lacking. Here we describe development application novel bacterial RNA amplification technology simultaneously identify key elements both pathogen responses a murine infection model. On side, found induction an unusual pattern stress response genes, host-induced metal ion limitation, failure achieve stationary phase vivo. mammalian observed surprising several genes encoding acute proteins including hepcidin, haptoglobin, complement C3 metallothionein 1 at site infection, as well other mediators innate immunity. Thus, our results reveal host–pathogen cross-talk not predicted by previous vitro analyses provide framework eavesdrop on broad array interactions As described here, comprehensive examination during critical discovery approaches for intervention current models.