Predictive model for inactivation of feline calicivirus, a norovirus surrogate, by heat and high hydrostatic pressure.

摘要: Noroviruses, which are members of the Caliciviridae family, represent leading cause nonbacterial gastroenteritis in developed countries; such norovirus infections result high economic costs for health protection. Person-to-person contact, contaminated water, and foods, especially raw shellfish, vegetables, fruits, can transmit noroviruses. We inactivated feline calicivirus, a surrogate nonculturable norovirus, cell culture medium mineral water by heat hydrostatic pressure. Incubation at ambient pressure 75°C 2 min as well treatment 450 MPa 15°C 1 more than 7 log10 PFU calicivirus per ml or water. The time-inactivation curves obtained with showed tailing logarithmic scale. Modeling nth-order kinetics virus inactivation was successful predicting infective particles. model enables prediction reduction response to pressures up 500 MPa, temperatures ranging from 5 75°C, various times. suggest processing foods reduce threat posed