Rapid-cycling Brassica Species: Inbreeding and Selection of B. campestris for Anther Culture Ability
作者： F. N. ASLAM , M. V. MACDONALD , P. LOUDON , D. S. INGRAM
DOI: 10.1093/OXFORDJOURNALS.AOB.A087969
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摘要: Since the base population of rapid-cycling B. campestris is self-incompatible, inbreeding was achieved by a combination budpollination and application pollen to cut surfaces decapitated stigmas. Three inbred generations were raised, in each generation plants selected for high or low potential anther embryogenesis. The proportion viable present anthers, as indicated fluorochromatic reaction germination test, also determined at stage. Lines with clearly defined embryogenesis isolated these experiments
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