Mast cell typing: demonstration of a distinct hematopoietic cell type and evidence for immunophenotypic relationship to mononuclear phagocytes.

摘要: The immunologic surface marker profile of human mast cells (MCs) was established using a combined toluidine/immunofluorescence staining procedure [49 monoclonal antibodies (MoAbs) tested]. Ascites (n = 9) MCs as well enzymatically dispersed from all organs tested (lung n 11, skin 7, intestinum 10) exhibited an identical profile. were recognized by MoAbs clustered CD9 (anti-gp24), CD33 (anti-gp67), and CD45 (anti-gp220) directed against membrane-bound IgE. MoAB YB5B8 (anti-gp145) selectively MCs. Most significantly, however, stained MAX1 (anti-gp65), MAX3 (anti-gp68), MAX11 MAX24 (anti-gp65). These bind to membrane antigens associated with late stage monocyte/macrophage differentiation. Thus, our results provide definite evidence that share markers mononuclear phagocytes. In contrast, are neither lymphatic (CD1-8, 10, 19-24) nor myelomonocytic (CD11-17). also lack the interleukin-2 (IL-2) receptor (CD25), T10 antigen (CD38), most myelocytic expressed on peripheral blood (PB) basophils. displayed unique phenotype within hematopoietic system. This new approach enabled us enrich lung purity greater than 95% means negative selection complement-mediated cell lysis. Purified subsequently analyzed flow cytometry, which confirmed obtained double-staining experiments. We next examined cultured metachromatic derived bone marrow (BM) colony-forming units (CFU). previously could not be classified morphologic criteria alone have therefore been termed basophil-like/MC-like cells. this study, toluidine blue-positive either pooled multipotential colonies (day 14-CFU-GEM) or 16/17-CFU-GM/G/M) MY7 (CD13), VIM12 (CD11b), VIM2, anti-IgE MoAb, after preincubation CFU-derived MoAb YB5B8. corresponds basophils excluded detectable formation mature in stem