Potential role of IL-10-treated dendritic cells in the control of the immune response to allergens

摘要: Several lines of evidence indicate that a defect in immunoregulatory mechanisms is involved the pathogenesis allergic asthma. The aim this study to determine whether IL-10-treated dendritic cells (DC) are able modulate allergen-specific T cell responses children affected by 41 (4-14 years) House Dust Mite (HDM), and 10 healthy age-matched were recruited. DC differentiated from peripheral blood CD14+ precursors cultured with GM-CSF IL-4 for 5 days. Der p2 (a major HDM allergen) was added alone or combination IL-10 48 hours obtain Dp2-DC IL10 Dp2-DC, respectively. Alternatively, presence pulsed during 2 last days culture (Dp2-DC10). ability resulting stimulate autologous promote anergy analyzed. Dp2-DC induced proliferation 32 out 41 patients but not controls. In 25 26 Dp2 DC10 significantly lower proliferation. The analysis phenotype showed treatment downregulated CD86 expression on Dp2-DC. However, no correlation between reduction observed. stimulation Th2 cytokine profile characterized an increase IL-5, IL-13 production IL-5/IFN-gamma ratio. same patients, co-culture both Dp2-DC10 caused marked IL-5 IL-13, parallel decrease Moreover, 8 we observed production. T generated Dp2-DC10, compared those generate hyporesponsive reactivation 4 tested, terms production: ratio. Our data show reduced stimulatory capacity through mechanism independent downregulation costimulatory signals. promoted suppression responses. associated production. These results represent important step forward prospective clinical application vivo.