Endothelial cell surface expression and binding of factor VIII/von Willebrand factor.

摘要: Factor VIII/von Willebrand factor (FVIII/vWF), a glycoprotein molecular complex found in human plasma, has been demonstrated by cell membrane fluorescence to be present on the surface of cultured umbilical cord vein endothelial cells. The nature these cells was established electron-microscopic studies that revealed presence Weibel-Palade bodies virtually all cultured. A newly developed radioreceptor assay used detect FVIII/vWF medium taken from cultures; concentration samples increased with time culture. binding showed no significant FVIII/vWF-specific surfaces and did not corroborate previous report suggesting receptor endothelium. membranes lack receptor-mediated suggests either absorbed non-specifically or is an integral part membrane.