Method and means for diagnosing tumors

作者: Mario Menschikowski , Gabriele Siegert , Albert Hagelgans

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摘要: The invention relates to a method and means for diagnosing tumors, in particular an early diagnosis (prevention) differentiating between benign malignant tumors using PCR, bodily fluids. according the is characterized by combination of pre-amplification process wherein methylated DNA sequences are amplified more strongly than non-methylated sequences, subsequent quantification special digital which significantly used normally prior art. As comparison data indicates, advantageously allows clear reliable conclusion as whether tumor disease present or not. suitable screening (prevention), monitoring progress disease, order exclude minimal residual (MRD), differential carcinoma tumors.

参考文章(19)
Xiwei Wu, Gerd P. Pfeifer, Tibor A. Rauch, Dna hypermethylation brain cancer markers ,(2011)
Louis Vermeulen, Jan Paul Medema, Felipe De Sousa E Melo, Methylation markers for colorectal cancer ,(2011)
Alexander Dobrovic, Lise Lotte Hansen, Tomasz Kazimierz Wojdacz, Method for detecting methylation status by using methylation-independent primers ,(2008)
Erin R. Chia, Amy L. Hiddessen, Stabilized droplets for calibration and testing ,(2013)
Arul M. Chinnaiyan, Mohan Saravana Dhanasekaran, Jung Kim, Dna methylation profiles in cancer ,(2012)
Mario Menschikowski, Uwe Platzbecker, Albert Hagelgans, Margot Vogel, Christian Thiede, Claudia Schönefeldt, Renate Lehnert, Graeme Eisenhofer, Gabriele Siegert, Aberrant methylation of the M-type phospholipase A 2 receptor gene in leukemic cells BMC Cancer. ,vol. 12, pp. 576- 576 ,(2012) , 10.1186/1471-2407-12-576
Tomasz K. Wojdacz, Lise Lotte Hansen, Reversal of PCR bias for improved sensitivity of the DNA methylation melting curve assay. BioTechniques. ,vol. 41, pp. 274- 278 ,(2006) , 10.2144/000112240
Steven T. Okino, Deepa Pookot, Shahana Majid, Hong Zhao, Long-Cheng Li, Robert F. Place, Rajvir Dahiya, Chromatin changes on theGSTP1 promoter associated with its inactivation in prostate cancer Molecular Carcinogenesis. ,vol. 46, pp. 839- 846 ,(2007) , 10.1002/MC.20313