The role of the dynamics of focal adhesion kinase in the mechanotaxis of endothelial cells.
作者： Song Li , Peter Butler , Yingxiao Wang , Yingli Hu , Dong Cho Han
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摘要: The migration of vascular endothelial cells (ECs) is critical in remodeling. We showed that fluid shear stress enhanced EC flow direction and called this “mechanotaxis.” To visualize the molecular dynamics focal adhesion kinase (FAK) at adhesions (FAs), FAK tagged with green fluorescence protein (GFP) was expressed ECs. Within 10 min application, lamellipodial protrusion induced cell periphery direction, recruitment FAs. ECs under migrated polarized formation new FAs these newly formed subsequently disassembled after rear moved over them. migrating had a decreased number In contrast to stress, serum did not significantly affect speed migration. Serum lamellipodia without directional preference. FAK(Y397) phosphorylation colocalized GFP-FAK both experiments. total level lower than treatment, suggesting change rather important for Our results demonstrate during response mechanical force, suggest mechanotaxis an mechanism controlling
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