Applications of azide-based bioorthogonal click chemistry in glycobiology.

摘要: Click chemistry is a powerful chemical reaction with excellent bioorthogonality features: biocompatible, rapid and highly specific in biological environments. For glycobiology, bioorthogonal click has created new method for glycan non-invasive imaging living systems, selective metabolic engineering, offered an elite handle manipulation glycomics studies. Especially the [3 + 2] dipolar cycloadditions of azides strained alkynes Staudinger ligation triarylphosphines have been widely used among extant reactions. This review focuses on azide-based chemistry, describing characteristics development these reactions, introducing some recent applications glycobiology research, especially including imaging, studies viral surface drug discovery as well other like activity-based protein profiling carbohydrate microarrays.