Hypotonic swelling of salicylate-treated cochlear outer hair cells

摘要: Abstract The outer hair cell (OHC) is a hydrostat with low hydraulic conductivity of Pf = 3 × 10−4 cm/s across the plasma membrane (PM) and subsurface cisterna that make up OHC’s lateral wall. SSC structurally functionally transport barrier in normal cells known to be disrupted by salicylate. effect sodium salicylate on Pf determined from osmotic experiments which isolated, control salicylate-treated OHCs were exposed hypotonic solutions constant flow chamber. value Pf = 3.5 ± 0.5 × 10−4 cm/s (mean ± s.e.m., n = 34) for was not significantly different Pf = 2.4 ± 0.3 × 10−4 cm/s n = 31) untreated (p = .3302). Thus PM unaffected treatment. ratio longitudinal strain radial ez/ec = −0.76 smaller (p = .0143) −0.72 OHCs, also independent magnitude applied challenge. Salicylate-treated took longer attain steady-state volume larger than increased 8–15% prior perfusion unlike α-ketoglutarate-treated OHCs. It suggested depolymerization cytoskeletal proteins and/or glycogen may responsible large increase as well responses modes application solution.