Development of a method for bioanalysis of a nanomaterial in biological matrices

摘要: Molecular imaging techniques have become an essential tool for cancer diagnosis. They the potential to detect at early stages and thus, they can change outcome of disease patient prognosis. Magnetic resonance (MRI) has arisen as one most promising methods used screening soft tumor tissues such breast cancer. However, specificity MRI remains poor leading misdiagnosis many false positive findings. Thus, a wide range new contrast agents (CAs) are being developed in order enhance image resolution safety For drugs with intended clinical use, it is necessary deep insight into viability molecule prior trials. Some vitro analysis vivo animal studies carried out characterize physicochemical properties compound its toxicity. Problems find right methodology assess identity nanoparticles after injected blood been previously found. In this project, combined method extract dummy particles, mimicking be CAs, was developed. These particles consist polymeric core, which contains metal ion inside, together coating attached surface. A approach characterization investigated by using three analytical techniques: gel permeation chromatography (GPC) separate size, enzyme-linked immunosorbent assay (ELISA) analyze inductively coupled plasma optical emission spectroscopy (ICP-OES) core ion. Different biological matrices, mainly serum urine, were tested prove identity. Results showed efficient extraction material high rate recovery GPC. Moreover, accuracy sensitivity ELISA ICP-OES detecting composition respectively proved. More importantly, extracted matrices demonstrated. Future required scale up further test samples from trials completely implement method. here first steps towards validation performed help understand changes upon exposure fluids body.