Porous chitosan bilayer membrane containing TGF-β1 loaded microspheres for pulp capping and reparative dentin formation in a dog model
作者： Fang Li , Xin Liu , Shouliang Zhao , Hong Wu , Hockin HK Xu
DOI: 10.1016/J.DENTAL.2013.11.005
关键词:
摘要: Abstract Objectives The objectives of this study were to develop a chitosan bilayer membrane containing microspheres with sustained TGF-β1 release enhance odontoblast-like cell function in vitro, and investigate pulp-capping dog model promote reparative dentin formation vivo for the first time. Methods A was synthesized consisting dense film on one side macroporous sponge other side. rationale use block perforated pulp from bacterial invasion, porous be loaded (MS-TGF) release. Pulp capping 48 teeth six beagle dogs performed test four groups: Control no material, commercial Dycal, without MS-TGF, MS-TGF. harvested histological analysis at two time points (10 60 d). Results spongy had macropores mean size 151 μm. porosity 83%. Chitosan showed release, gradually releasing 42% 7 d. proliferation cells MS-TGF much greater than that TGF (p Significance novel bilayer-microsphere construct generated 3–6 times more controls growth factor delivery may useful wide range dental regenerative medicine applications.
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