The control of epidermal growth factor grafted on mesoporous silica nanoparticles for targeted delivery

摘要: The performance of biomaterials in a biological environment is largely influenced by the surface properties biomaterials. In particular, grafted targeting ligands significantly impact subsequent cellular interactions. utilisation epidermal growth factor (EGF) effective for targeted delivery drugs to tumours, but amount these attachments cannot be easily quantified as most characterization methods could not detect extremely low EGF on nanoparticles. this study, hollow mesoporous silica nanoparticles (HMSNs) were functionalized with amine groups conjugate EGFs via carbodiimide chemistry. Time flight secondary ion mass spectrometry (ToF-SIMS), very specific technique (penetration depth <1.5 nm), was employed study binding efficiency Principal component analysis (PCA) implemented track relative concentrations HMSNs. It found that ToF-SIMS combined PCA an method evaluate immobilization EGFs. Based useful technique, quantity and density can effectively controlled varying concentration at grafting stages. Cell experiments demonstrated EGFR positive cells affected number attached