Heterogeneity of Crystalline Rennin

作者: C.A. Ernstrom

DOI: 10.3168/JDS.S0022-0302(58)91150-0

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摘要: Summary Block-shaped crystals were prepared from a concentrated solution of purified rennin by dialyzing it against Berridge's salting-in buffer. The purification procedure was relatively simple, and proved successful in each several attempts to obtain pure enough crystallize. A single boundary electrophoresis represented over 96% the protein when dissolved run at pH 6.8 sodium phosphate buffer an ionic strength 0.2. However, least four components evident same 0.033 strength. These results show that preparations can not be considered homogeneous on basis their crystalline form or electrophoretic behavior buffers high

参考文章(23)
N. J. Berridge, Rennin and the clotting of milk. Advances in enzymology and related subjects of biochemistry. ,vol. 15, pp. 423- 448 ,(2006) , 10.1002/9780470122600.CH10
William J. Dreyer, Hans Neurath, The activation of chymotrypsinogen; isolation and identification of a peptide liberated during activation. Journal of Biological Chemistry. ,vol. 217, pp. 527- 539 ,(1955) , 10.1016/S0021-9258(18)65918-6
N. J. Berridge, The purification and crystallization of rennin. Biochemical Journal. ,vol. 39, pp. 179- 186 ,(1945) , 10.1042/BJ0390179
H. Schwander, P. Zahler, Hs. Nitschmann, Das Lab und seine Wirkung auf das Casein der Milch. V. Analytische Untersuchungen an kristallisiertem Lab Helvetica Chimica Acta. ,vol. 35, pp. 553- 560 ,(1952) , 10.1002/HLCA.19520350220
H.H. Sommer, Helene Matsen, The Relation of Mastitis to Rennet Coagulability and Curd Strength of Milk Journal of Dairy Science. ,vol. 18, pp. 741- 749 ,(1935) , 10.3168/JDS.S0022-0302(35)93196-4
Robert L. Baldwin, Paul M. Laughton, Robert A. Alberty, Homogeneity and the electrophoretic behavior of some proteins. III A general method for the determination of mobility distributions. The Journal of Physical Chemistry. ,vol. 55, pp. 111- 125 ,(1951) , 10.1021/J150484A013
C.L. Hankinson, The Preparation of Crystalline Rennin Journal of Dairy Science. ,vol. 26, pp. 53- 62 ,(1943) , 10.3168/JDS.S0022-0302(43)92693-1
Robert A. Alberty, An introduction to electrophoresis: Part II: Analysis and theory. Journal of Chemical Education. ,vol. 25, pp. 619- ,(1948) , 10.1021/ED025P619