Identification of Functional Elements in the Promoter Region of the Human Gene for Thymidylate Synthase and Nuclear Factors That Regulate the Expression of the Gene

摘要: To identify the essential motifs of promoter human gene for thymidylate synthase (TS), we constructed a set deletion mutants from 5′-terminal region TS gene. From results assays expression chloramphenicol acetyltransferase (CAT), identified two functional elements with positive effects on activity: CACCC box (CCACACCC) and an Sp1-binding motif (GAGGCGGA) that was homologous to site in mouse In addition, negative regulatory sequences were between upstream box. The gel mobility shift analyses suggested Sp1 binds multiple nuclear factors are related bind Furthermore, binding mutated correlated activity, as measured by CAT assay. Therefore, seems be major contributor basic activity gene, although involved regulated this