Improving selectivity in multimodal chromatography using controlled pH gradient elution.

摘要: Externally generated pH gradients are employed on a multimodal cation exchange chromatographic resin to improve the selectivity for mixture of model proteins. By combining controlled with unique selectivities arising from multiple interaction types exhibited by resin, separation protein is significantly improved as compared linear salt gradient operation. Several conditions explored and shallow 3.8 5.5 shown be able resolve This work provides proof concept use in chromatography sets stage future applications.