Separation of protein mixtures using pH-gradient cation-exchange chromatography.

摘要: Historically, separation of a protein mixture after adsorption to cation-exchange column is effected by alteration in ionic strength. An alternative method using pH induced gradient the range 4-7.5 was studied. A with large particle beads containing excessive carboxyls employed. across generated step change at entrance non-retained buffer system. Consistency and accuracy values timed intervals were demonstrated three different batches. In development application, we found correlation coefficient >0.9 between elution six acidic proteins their isoelectric points. One case study showed resolution monoclonal antibody species from column. Another feasibility separating polyethylene glycol conjugated native protein.