Prevalence and characterization of Salmonella serovars isolated from farm products in Shanghai

作者: Pei’en Ni , Qian Xu , Yujie Yin , Danlei Liu , Jvmei Zhang

DOI: 10.1016/J.FOODCONT.2017.10.009

关键词:

摘要: Abstract Salmonella is a major bacterial pathogen that causes foodborne illness worldwide. Farm products, including raw meat, vegetables, and dairy may be important vehicles for transmitting pathogens, thus representing threat to human health. In this study, the prevalence of isolated from farm products in Shanghai was investigated characterized. A total 344 samples were randomly collected farmers’ markets, supermarkets, milk factories, slaughterhouse between March 2016 February 2017. Using Chinese National Standard method (GB 4789.4–2010), 83 (24.1%) determined positive Salmonella, 42 (42.4%) pork, 21 (41.2%) chicken, 15 (31.3%) beef, 3 (16.7%) mutton, 1 (4.5%) lettuce, (3.8%) cucumber. All egg negative Salmonella. Ninety strains samples, 12 serovars identified, Enteritidis (n = 35), Typhimurium (n = 22), Derby (n = 13), Anatum (n = 5), Agona (n = 4), Thompson (n = 3), Indiana Braenderup (n = 1), Heidelberg Infantis Litchfield (n = 1) Manhattan (n = 1). isolates characterized by multilocus sequence typing (MLST) subtyped pulsed-field gel electrophoresis (PFGE). Thirteen types (ST) among which ST11, ST34, ST40 predominant STs. ST highly correlated with serovar type. The results PFGE showed 73 patterns generated isolates. conclusion, contamination common Shanghai, diversity high

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