Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet.
作者： Efrain M. Ribot , M.A. Fair , R. Gautom , D.N. Cameron , S.B. Hunter
关键词:
摘要: Standardized rapid pulsed-field gel electrophoresis (PFGE) protocols for the subtyping of Escherichia coli O157:H7, Salmonella serotypes, and Shigella species are described. These used by laboratories in PulseNet, a network state local health departments, other public that perform real-time PFGE these bacterial foodborne pathogens surveillance outbreak investigations. Development standardization consisted thorough optimization reagents reaction conditions to ensure yielded consistent results high-quality pattern data all PulseNet participating laboratories. based on original 3–4-day standardized procedure developed at Centers Disease Control Prevention was validated 1996 1997 eight independent By using protocols, able subtype pathoge...
liebertonline.com参考文章(36)
Mary Elizabeth Kaufmann, Pulsed‐Field Gel Electrophoresis Methods in molecular medicine. ,vol. 15, pp. 33- 50 ,(1998) , 10.1385/0-89603-498-4:33
D. Tapalski, R.S. Hendriksen, H. Hasman, P. Ahrens, F.M. Aarestrup, Molecular characterisation of multidrug-resistant Salmonella enterica serovar Typhimurium isolates from Gomel region, Belarus Clinical Microbiology and Infection. ,vol. 13, pp. 1030- 1033 ,(2007) , 10.1111/J.1469-0691.2007.01795.X
S D Holmberg, I K Wachsmuth, F W Hickman-Brenner, M L Cohen, Comparison of plasmid profile analysis, phage typing, and antimicrobial susceptibility testing in characterizing Salmonella typhimurium isolates from outbreaks. Journal of Clinical Microbiology. ,vol. 19, pp. 100- 104 ,(1984) , 10.1128/JCM.19.2.100-104.1984
T J Barrett, H Lior, J H Green, R Khakhria, J G Wells, B P Bell, K D Greene, J Lewis, P M Griffin, Laboratory investigation of a multistate food-borne outbreak of Escherichia coli O157:H7 by using pulsed-field gel electrophoresis and phage typing. Journal of Clinical Microbiology. ,vol. 32, pp. 3013- 3017 ,(1994) , 10.1128/JCM.32.12.3013-3017.1994
D. Michael Olive, Pamela Bean, Principles and Applications of Methods for DNA-Based Typing of Microbial Organisms Journal of Clinical Microbiology. ,vol. 37, pp. 1661- 1669 ,(1999) , 10.1128/JCM.37.6.1661-1669.1999
Annaëlle Kérouanton, Muriel Marault, Renaud Lailler, François-Xavier Weill, Carole Feurer, Emmanuelle Espié, Anne Brisabois, Pulsed-field gel electrophoresis subtyping database for foodborne Salmonella enterica serotype discrimination. Foodborne Pathogens and Disease. ,vol. 4, pp. 293- 303 ,(2007) , 10.1089/FPD.2007.0090
J. N. Maslow, M. E. Mulligan, R. D. Arbeit, Molecular Epidemiology: Application of Contemporary Techniques to the Typing of Microorganisms Clinical Infectious Diseases. ,vol. 17, pp. 153- 162 ,(1993) , 10.1093/CLINIDS/17.2.153
Hsiao-Lun Wei, You-Wen Wang, Chun-Chin Li, Sheng Kai Tung, Chien-Shun Chiou, Epidemiology and evolution of genotype and antimicrobial resistance of an imported Shigella sonnei clone circulating in central Taiwan Diagnostic Microbiology and Infectious Disease. ,vol. 58, pp. 469- 475 ,(2007) , 10.1016/J.DIAGMICROBIO.2007.03.006
Robert Tauxe, Emerging Foodborne Diseases: An Evolving Public Health Challenge Emerging Infectious Diseases. ,vol. 3, pp. 425- 434 ,(1997) , 10.3201/EID0304.970403
Jean M. Whichard, Kathryn Gay, Jennifer E. Stevenson, Kevin J. Joyce, Kara L. Cooper, Michael Omondi, Felicita Medalla, George A. Jacoby, Timothy J. Barrett, Human Salmonella and concurrent decreased susceptibility to quinolones and extended-spectrum cephalosporins. Emerging Infectious Diseases. ,vol. 13, pp. 1681- 1688 ,(2007) , 10.3201/EID1311.061438