Selective Expansion of Alveolar Macrophages In Vivo by Adenovirus-Mediated Transfer of the Murine Granulocyte-Macrophage Colony-Stimulating Factor cDNA

摘要: Based on the hypothesis that genetic modification of freshly isolated alveolar macrophages (AM) with granulocytemacrophage colony-stimulating factor (GM-CSF) cDNA would induce AM to proliferate, this study focuses ability adenoviral (Ad) vectors transfer and efficiently express murine (m) GM-CSF in consequent expansion number vitro vivo. To demonstrate an Ad vector can effectively genes AM, recovered by bronchoalveolar lavage from lung Balb/c mice were infected coding for green fluorescent protein (GFP) expressed GFP a dose-dependent fashion. Infection containing expression cassette mGM-CSF led proliferation vitro. When AdGFP returned respiratory tract syngeneic recipient mice, GFP-expressing cells could still be 2 weeks later. In infection AdmGM-CSF subsequent transplantation genetically modified lungs recipients Strikingly, 5 after demonstrated increased rate proliferation, total was 1.9-fold greater than controls. Importantly, increase numbers selective (ie, other inflammatory cell unchanged), there no architecture. Thus, it is feasible modify use strategy behavior importance primary defense epithelial surface, may useful enhancing pulmonary defenses immunodeficiency states. r 1999 The American Society Hematology.