The influence of substrate and electron acceptor availability on bioactive zone dynamics in porous media.

作者: Irfan Yolcubal , Jonathan G. Dorn , Raina M. Maier , Mark L. Brusseau

DOI: 10.1016/S0169-7722(03)00027-5

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摘要: Two approaches were used to investigate the influence of dissolved oxygen (DO) and substrate availability on formation dynamics ‘‘bioactive zones’’ in a water-saturated porous medium. A bioactive zone is defined as region where microbial community sufficiently active metabolize bioavailable substrates. In first approach, activity was characterized by monitoring spatial temporal variability DO aqueous (salicylate naphthalene) concentrations during miscible-displacement experiments. second monitored using multiple fiber optics emplaced medium detect luminescence produced Pseudomonas putida RB1353, bioluminescent reporter organism that produces light when salicylate (an intermediate naphthalene degradation) present. The results both show location size zones influenced situ availability. When not limiting factor (i.e., lower input concentrations), encompassed entire column, with majority occurring between inlet midpoint. However, became for higher

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