The influence of system complexity on bacterial transport in saturated porous media

摘要: Abstract A series of miscible-displacement column experiments were conducted under saturated flow conditions to systematically investigate the influence physical and biological complexity on bacterial activity fate in presence absence a non-sorbing growth substrate, salicylate. Bacterial elution was monitored for three different systems; System I—a sterilized, inoculated, well-sorted sand, II—a heterogeneous loamy sand (Hayhook), III—two unsterilized sands (Hayhook Vinton) each with their associated indigenous microbial community. Results show that I behaved ideally respect both cell substrate transport, wherein: (1) occurred response addition, (2) increased pulse, (3) breakthrough curves reproducible elution. In contrast, II showed ideal behavior transport but variable transport. Further, there no measurable addition increase during salicylate pulse. III exhibited non-ideal Of particular interest is fact communities two soils differently. Specifically, Hayhook soil, an observed heterotrophic population while salicylate-degrading community preferentially retained column. contrast Vinton pulse did not elicit from either or culturable, microorganisms. For Systems III, variability appears be component system, since sterile controls reproducible. This type systematic study critical understanding complex, systems, illustrates potential uncertainty measurements such systems.