The release of acetylcholine from carotid body tissues. Further study on the effects of acetylcholine and cholinergic blocking agents on the chemosensory discharge

摘要: 1. Both carotid bodies were removed from cats and placed in a small Perspex channel through which Locke solution was allowed to flow under layer of paraffin oil. Stimulation the upstream (‘donor’) organ elicited an increased sensory discharge downstream (‘detector’) preparation (Loewi effect). 2. This effect enhanced by eserine, depressed hexamethonium blocked either mecamylamine or acetylcholinesterase. The Loewi did not disappear when chronically denervated (4 days) ‘donor’ stimulated. 3. These experiments led conclusion that ACh is released body tissues (probably glomus cells) during stimulation this substance responsible for initiation chemosensory discharges. 4. In single preparations endings proved be very sensitive especially presence eserine which, all probability, inactivated tissue cholinesterase. Curarizing agents such as mecamylamine, hexamethonium, (+)-tubocurarine atropine response applied what appeared ‘surmountable’ antagonism.