Structural studies of DNA through cleavage by the hydroxyl radical.

摘要: Although the overall architecture of DNA has been clear since work Watson and Crick, the-detailed structure (and its dependence on sequence) is still an area active research. The chief experimental difficulty that as it exists in biological systems a very large molecule. traditional methods for chemical determination (for example, X-ray crystallography NMR), while able to provide important insights into structures small oligonucleotides, are at loss when structural information needed much larger natural molecules.