Diversity of Capsular Polysaccharide Gene Clusters in Kpc-Producing Klebsiella pneumoniae Clinical Isolates of Sequence Type 258 Involved in the Italian Epidemic

摘要: Strains of Klebsiella pneumoniae producing KPC-type beta-lactamases (KPC-Kp) are broadly disseminating worldwide and constitute a major healthcare threat given their extensively drug resistant phenotypes ability to rapidly disseminate in settings. In this work we report on the characterization two different capsular polysaccharide (CPS) gene clusters, named cpsBO-4 cps207-2, from KPC-Kp clinical strains Italy belonging sequence type (ST) 258, which is one most successful ST spreading worldwide. While was known 78 K-types according recently proposed typing schemes based wzi or wzc sequences, cps207-2 classified as K41 by these methods. Bioinformatic analysis revealed that they were represented genomic sequences ST258 countries, also detected strain ST442 Brazil. Investigation collection 46 ST512 (a single locus variant ST258) representative recent Italian epidemic means multiplex PCR approach prevalent type, being both with countrywide distribution, while only more restricted distribution.