An amperometric sensor employing glucose oxidase immobilized on nylon membranes with different pore diameter and grafted with different monomers
作者： M. Portaccio , M. El-Masry , N. Rossi Diano , A. De Maio , V. Grano
DOI: 10.1016/S1381-1177(02)00058-9
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摘要: Abstract Glucose oxidase (GOD) was immobilized on nylon membranes having three different pore diameters and chemically grafted with glycidyl methacrylate (GMA) or butyl (BMA). Hexamethylenediamine (HMDA) glutaraldehyde (GA) were used as spacer coupling agent, respectively. The biochemical electrochemical behaviour of the has been studied a function pH, temperature glucose concentration reference to monomer membrane diameter. soluble GOD also in order see modification induced by immobilization process enzyme activity. It found that values biosensor sensitivity, maximum saturation current affinity increase diameter, indipendently nature graft monomer. Opposite relatively extension linear response ranges average times. With parameters increasing diameter it GMA had higher than those BMA. contrary occurred decreasing Biochemical results have discussed terms limitations diffusion substrate reaction products across catalytic introduced hydrophobicity monomers.
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