An optical glucose biosensor based on glucose oxidase immobilized on a swim bladder membrane
作者： Zaide Zhou , Lina Qiao , Peng Zhang , Dan Xiao , Martin M. F. Choi
DOI: 10.1007/S00216-005-0023-8
关键词:
摘要: An optical glucose biosensor using a swim bladder membrane as an enzyme immobilization platform and oxygen-sensitive oxygen transducer has been developed. During the enzymatic reaction, is oxidized by oxidase with concomitant consumption of dissolved resulting in increase fluorescence intensity transducer. The directly related to concentration. effects pH, temperature, buffer concentration, selectivity have studied detail. immobilized retained 80% its initial activity after being kept for more than 10 months at 4°C. successfully applied determination content human blood serum urine samples.
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