The construction of glucose biosensor based on crystalline iridium(III)-containing coordination polymers with fiber-optic detection
作者： Mei-Lin Ho , Jing-Chang Wang , Ting-Yi Wang , Chun-Yen Lin , Jian Fan Zhu
DOI: 10.1016/J.SNB.2013.08.100
关键词: Detection limit 、 Substrate (chemistry) 、 Inorganic chemistry 、 Glucose oxidase 、 Polymer 、 Phosphorescence 、 Biosensor 、 Matrix (chemical analysis) 、 Luminescence 、 Analytical chemistry 、 Chemistry
摘要: Abstract This study reports a simple and sensitive method for phosphorescence detection of glucose concentration in beverage samples human serum. optical sensor was constructed based on sensing substrate made crystalline iridium(III)-containing coordination polymers doped sol–gel matrix built up under oxidase encapsulated hydrogel then immobilized egg membrane, using the layer-by-layer method. The mechanism is depletion oxygen content upon exposure solution with concomitant increase polymers. effects buffer concentration, pH, interference temperature have been studied detail. Under optimum conditions, luminescence changing rate revealed good linear relationship from 0.05 mM to 5.0 mM ( y = 1.058 [glucose] + 0.6591, R 2 = 0.9935) limit 0.01 mM. has also successfully applied determination real samples.
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