Glucose sensing based on the intrinsic fluorescence of sol-gel immobilized yeast hexokinase.

摘要: In this study, we investigated measurements of the intrinsic fluorescence yeast hexokinase as an assay for glucose and immobilization enzyme in a silica sol-get matrix potential vivo sensor use patients with diabetes. The solution (excitation = 295 nm, emission 330 nm) decreased by 23% at saturating concentration 1 mM (K-d 0.3 mM), but serum abolished glucose-related response. When entrapped tetramethylorthosilicate-derived sol gel, retained activity, 25% maximal decrease fluorescence, saturation point was increased to 50 12.5 mM). response range further (to 120 mM, K-d 57 mM) covering membrane poly(2-hydroxyethyl) methacrylate. Unlike free enzyme, responses sol-gel immobilized hexokinase, or Without membrane, were similar buffer serum. We conclude that monitoring is Suitable system development biosensor. (c) 2005 Elsevier Inc. All rights reserved.