Enzyme for Biosensing Applications

摘要: Enzymes are very efficient biocatalysts, which have the ability to specifically recognize their substrates and catalyze transformation. These unique properties make enzymes powerful tools develop analytical devices. Enzyme-based biosensors associate intimately a biocatalyst-containing sensing layer with transducer. The transformations catalyzed by an enzyme come variations of some physicochemical parameters. role transducer is convert those signals into measurable electrical signal. In biosensors, generally immobilized on or close Depending chemical physical characteristics support, different immobilization techniques can be implemented. transduction mode will adapted parameter that monitored. variation concentration substrate product in course enzymatic reaction detected help sensor, then acts as Electrochemical been developed involving oxidoreduction reactions. Optical based either fluorescence, absorbance, bioluminescence chemiluminescence measurements. Enzymatic reactions usually associated high enthalpy change, results temperature recorded using thermistor. Gravimetric mass induced reaction.